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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has actually been noticed in many tumors, which results in chromosome instability, and so may possibly contribute to tumorigenesis. This protein was found to affiliate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in cell-cycle regulated histone gene expression and performs a vital function in promoting mobile-cycle progression during the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are already described. Two more splice variants have been reported but specific nucleotide sequence details is not really nevertheless accessible.
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Blocking peptides are peptides that bind specially to the goal antibody and block antibody binding. These peptide ordinarily consists of the epitope identified via the antibody.
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This manual outlines further probable optimization of mobile and immunoassay parameters to make certain the best possible success are...
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This tutorial outlines further more possible optimization of mobile and immunoassay parameters to ensure the very best results are...
Antibodies bound to the blocking peptide now not bind to the epitope over the concentrate on protein. By evaluating the staining with the blocked antibody versus the antibody on your own, you can see which staining is unique.
Target Information Cyclin E belongs to the really conserved cyclin spouse and children, whose customers are characterized by a dramatic periodicity in protein abundance in the mobile cycle. Cyclins operate as regulators of CDK kinases. Distinct cyclins exhibit unique expression and degradation patterns which contribute towards the temporal coordination of each and every mitotic event. Cyclin E forms a posh with and features for a regulatory subunit of CDK2, whose exercise is needed for mobile cycle G1/S changeover. This protein accumulates in the G1-S section boundary which is degraded as cells progress as a result of S period.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of read more receptor activation by both of those antibodies...
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